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. 2020 Dec 11;12(12):3717. doi: 10.3390/cancers12123717

Figure 6.

Figure 6

Effect of BMS-754807 and OSI-906 on the activation of off-target protein kinases. The RWP-1 pancreatic carcinoma cell line was treated for 1 or 6 h with 10 µM BMS-754807 or OSI-906. ERK ½ (A), JNK 1, 2, 3 (B) and p38α (C) were determined by using an InstantOne ELISA kit from eBioscience. (D). RWP-1 cells were treated with 10 µM BMS-754807 or OSI-906, or left untreated for 6 h, and then subjected to analysis on a human phosphor-RTK array. The graph shows the effect of both inhibitors on the phosphorylation status of the different protein kinases included in the array. Data represent the mean ± SEM (n ≥ 3) of phosphorylation levels, taking those of untreated cells as 100%. *, p < 0.05; **, p < 0.01.