Skip to main content
. 2020 Nov 20;432(23):6075–6091. doi: 10.1016/j.jmb.2020.10.008

Figure 1.

Figure 1

TET1 contains independent NANOG-binding regions. (a) and (b) Co-immunoprecipitations of non-overlapping (Flag)3-TET1 N- and C-terminal constructs (a) with (HA)3-NANOG from E14/T ESCs. (b) Immunoblots were probed with the antibodies indicated on the left (representative images, n = 2). (c) and (d) Co-purification of MBP-tagged TET1 N-terminal constructs with (His)6-NANOG from E. coli. (c) Fragments of the TET1 N-terminus are shown in the context of full length TET1. (d) Immunoblots were probed with the antibodies indicated on the left (representative images, n = 3). (e) and (f) Co-purification of (His)6-NANOG with alanine substitution mutants of MBP-TET1 (1–120) from E. coli. (e) Alanine substitution mutants of TET1 (109–120) are shown in the context of full length TET1. (f) Immunoblots were probed with the antibodies indicated on the left (representative images, n = 3).