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. 2020 Nov 20;432(23):6075–6091. doi: 10.1016/j.jmb.2020.10.008

Figure 3.

Figure 3

The NANOG interaction domain 2 of TET1 binds NANOG using phenylalanine residues. (a) and (b) Co-immunoprecipitation of the (Flag)3-TET1 constructs (a) with (HA)3-NANOG from E14/T ESCs. (b) Immunoblots were probed with the antibodies indicated on the left (representative images, n = 3). (c) and (d) Co-immunoprecipitation of alanine substitution mutants of (Flag)3-TET1(734–1169) with (HA)3-NANOG from E14/T ESCs. (c) Alanine substitutions of the amino acids within residues 1156–1169. (d) Immunoblots were probed with the antibodies indicated on the left (representative images, n = 2).