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. 2020 Dec 12;12(12):1428. doi: 10.3390/v12121428

Figure 4.

Figure 4

Correct sorting is necessary for cleavage of gG2. (A) Schematic representation of the SgG2 and the SgG2ΔSP constructs. Indicated amino acid positions are based on HSV-2 strain 333 gG2 WT sequence. (B) Western blots showing detection of gG2 proteins in the cell lysate (CL, left and middle blot) and supernatant (SN, right blot) of HEK-293T cells transfected with SgG2 or SgG2ΔSP. The left panel shows green and red channel in composite image, the middle blot shows only the HA-tag signal in the red channel for the cell lysate samples. The proteins were detected with anti-V5 (green) and anti-HA (red) antibodies. Black arrowheads indicate position of SgG2 cleavage products, white arrowhead indicates precursor proteins. Black arrow indicates unprocessed product of SgG2ΔSP. One of three independent experiments is shown. (C) Bioinformatics online tool ProP 1.0 [25] was used to analyse the sequence of gG2 for general propeptide convertase (PC) cleavage motifs. Schematic representation of gG2 domains in relation to detected motifs. Motif with the highest score at Arg321 is indicated. Indicated amino acid positions are based on HSV-2 strain 333 gG2 WT sequence.