Figure 5.

Mutations of Arg321 and Arg341 do not completely abrogate cleavage. (A) Schematic representation of SgG2 and derived constructs. The wild type CS sequence (amino acids 314–343) and mutated residues (in red) are shown. Underlined residues indicate potential furin motifs; vertical lines in sequence indicate proposed cleavage. (B) Western blots showing gG2 protein detected with anti V5 or HA antibodies in the cell lysate (CL, left blot) and supernatant (SN, right blot) of HEK-293T transfected cells. Black arrowhead indicates SgG2 cleavage products, and white arrowheads indicate precursor protein. One of four independent experiments is shown.