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. 2020 Dec 9;21(24):9379. doi: 10.3390/ijms21249379

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knockout of Igκ induced mitochondria-mediated apoptosis in the NCTC1469 cell line. NCTC1469 cells were transfected with si-Igκ or control si-RNA (NC) for 24 h before analysis. (a) Bright-field images and immunofluorescence staining of Igκ. (b) Igκ knockout promoted NCTC1469 cell apoptosis on Annexin V and 7-AAD analysis. (c) Igκ knockout in NCTC1469 cells reduced mitochondrial membrane potential. (d) Protein mass spectrometry analysis of NCTC1469 cells transfected with si-Igκ and NC. (e) Western blot analysis of cleaved poly (ADP-ribose) polymerase, caspase-3, cleaved caspase-3, Igκ, cleaved caspase-9, cytochrome c, p65, p-p65, p-JNK, JNK and β-actin after Igκ knockout in NCTC1469 cells.