Figure 5.
(a) Proliferation, (b) metabolic activity, and (c) morphology of VSMCs cultured on PS culture dishes coated with PP films, deposited at the average RF power (Pav) of 10, 33, and 150 W (labelled as CPA-10, CPA-33, and CPA-150, respectively), evaluated within 7 days of cultivation. (a,b) The dots represent individual biological samples. Each dot depicts the arithmetic mean calculated (a) from cell numbers obtained from a minimum of 15 pictures per biological sample or (b) from three pipetting replicates per biological sample. The bold central lines show (a) the arithmetic mean and (b) the median of biological samples. Statistically significant differences (p ≤ 0.05) are marked by horizontal lines with asterisks connecting the differing samples. (c) Images of the cells were captured 7 days after seeding by an epifluorescence Olympus IX-71 microscope. Cell nuclei are visualized by Hoechst #33258 (blue), while the cell membrane and the actin cytoskeleton are visualized by Phalloidin-TRITC (red). The scale bar depicts 100 µm.