Table 2.
List of the peptides identified in the partially purified peptide fraction obtained from the fermented hemp through RP-FPLC. Fermentation was carried out by Lactiplantibacillus plantarum 18S9 and Leuconostoc mesenteroides 12MM1 (ratio 1:1, final cell density of circa 107 cfu/g) at 30 °C for 24 h.
| Fraction | Sequence | Weight (Da) |
Length (aa) | Net Charge | Hydrophobic-AA (%) | BCAA * (%) |
NCBI Accession n° (Protein) | Fragment |
|---|---|---|---|---|---|---|---|---|
| 1 | ALASIGKATR | 986.6 | 10 | 2 | 60 | 20 | XP_030502118.1 (nephrocystin-3) | 233–242 |
| 2 | IGQSHPQALMYPLLVACKSISNLR | 2638.4 | 24 | 3 | 52 | 28 | XP_030508478.1 (Serine/threonine-protein kinase TOR) | 1863–1886 |
| 5 | AQVSVGGGR | 829.4 | 9 | 2 | 66 | 22 |
XP_030499964.1 (edestin 3) |
119–127 |
| 7 | AIENGAVSVSEPEEK | 1557.7 | 15 | 2 | 44 | 19 |
XP_030503904.1 (uncharacterized protein) |
99–113 |
| DLQIIAPSR | 1011.5 | 9 | 2 | 55 | 33 |
SNQ45155.1 (edestin 3) |
286–194 |
* BCAA, branched-chain amino acids.