Table 1.
Effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benziladenine (BA) on diameter (cm) and fresh mass weight (g/callus) of calli induced in nodal segments of Phyllanthus amarus.
| 2,4-D | Fresh Weight | BA/Callus Diameter (cm) Mean ± SD | ||
|---|---|---|---|---|
| (mg/L) | (g/callus) Mean ± SD |
0 mg/L | 1 mg/L | 2 mg/L |
| 0 | 0.00 ± 0.00 c | 0.00 ± 0.00 cA | 0.00 ± 0.00 cA | 0.00 ± 0.00 bA |
| 0.25 | 2.00 ± 0.23 a | 2.58 ± 0.30 aA | 2.00 ± 0.30 aB | 1.76 ± 0.37 aB |
| 0.50 | 1.41 ± 0.30 b | 2.23 ± 0.37 bA | 1.59 ± 0.21 bB | 1.57 ± 0.04 aB |
| 0.75 | 1.24 ± 0.20 b | 1.99 ± 0.58 bA | 1.43 ± 0.20 bB | 1.56 ± 0.29 aB |
| F (2,4-D) 1 | 103.07 ** | 294.05 ** | ||
| F (BA) | 5.57 ** | 31.10 ** | ||
| F (2,4-D × BA) | 0.94 ns | 4.39 ** | ||
| CV (%) | 11.55 | 21.73 | ||
1 Each mean represents 10 replicates (test tubes) with one nodal segment each. Means within a column (2,4-D effects; lowercase) or lines (BA effects; uppercase) followed by different letters are significantly different according to the Scott-Knot test at 1% (**) level of probability. All data followed a normal distribution according to the Shapiro-Wilk test. SD = standard deviation of means; F = distribution of variances according the F test; CV = coefficient of variation; ns = not significant.