Table 2.
Effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benziladenine (BA) on diameter (cm) and fresh mass weight (g/callus) of proliferated calli of Phyllanthus amarus.
| 2,4-D | BA | BA | ||||
|---|---|---|---|---|---|---|
| (mg/L) | 0 mg/L | 1 mg/L | 2 mg/L | 0 mg/L | 1 mg/L | 2 mg/L |
| Callus Diameter (cm) Mean ± SD | Fresh Weight (g) Mean ± SD | |||||
| 0.00 | 1.01 ± 0.23 cA | 1.11 ± 0.36 bA | 1.09 ± 0.41 dA | 0.17 ± 0.31 bA | 0.32 ± 0.24 bA | 0.30 ± 0.41 bA |
| 0.25 | 1.05 ± 0.26 cB | 2.03 ± 0.26 aA | 2.17 ± 0.56 aA | 0.16 ± 0.37 bB | 1.79 ± 0.34 aA | 2.01 ± 0.24 aA |
| 0.50 | 2.39 ± 0.28 aA | 1.85 ± 0.29 aB | 1.73 ± 0.31 bB | 1.89 ± 0.44 aA | 1.29 ± 0.28 aA | 1.37 ± 0.44 aA |
| 0.75 | 2.04 ± 0.41 bA | 1.66 ± 0.58 aB | 1.50 ± 0.17 cB | 2.30 ± 0.33 aA | 1.15 ± 0.38 aB | 0.86 ± 0.31 bB |
| F (2,4-D) 1 | 35.30 ** | 31.97 ** | ||||
| F (BA) | 0.16 ns | 0.31 ns | ||||
| F (2,4-D × BA) | 14.35 ** | 14.34 ** | ||||
| CV (%) | 24.39 | 14.34 | ||||
1 Each mean represents 10 replicates (test tube) with one nodal segment each. Means within a column (2,4-D effects; lowercase) or lines (BA effects; uppercase) followed by different letters are significantly different according to the Scott-Knott test at the 1% (**) level of probability. All data followed a normal distribution according to the Shapiro-Wilk test. SD = standard deviation of means; F = distribution of variances according the F test; CV = coefficient of variation; ns = not significant.