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. 2020 Dec 12;25(24):5895. doi: 10.3390/molecules25245895

Table 4.

Effects of the elicitors chitosan and salicylic acid (SA) on in vitro callus growth under darkness + 2,4-D and light + 6-BA.

Elicitors 1 0.5 mg/L 2,4-D + Darkness 2.0 mg/L BA + Light
Diameter (cm)
Mean ± SD
Fresh Weight (g/callus)
Mean ± SD
Fresh Weight (g/callus)
Mean ± SD
Control 0.50 ± 0.24 a 1.51 ± 0.45 a 1.78 ± 0.41 a
Salicylic acid 0.35 ± 0.38 a 0.90 ± 0.38 c 1.83 ± 0.33 a
Chitosan 0.40 ± 0.44 a 1.31 ± 0.36 b 1.77 ± 0.48 a
F 1 0.33 ns 24.59 ** 0.07 ns
CV (%) 13.17 15.89 21.16

1 Each mean represents 10 replicates (test tube) with one nodal segment each. Means within a column followed by the different letters are significantly different according to the Scot-Knott test at the 1% (**) level of probability. All data followed a normal distribution according to the Shapiro–Wilk test. F = distribution of variances according the F test; CV = coefficient of variation; SD = standard deviation of means; ns = not significant.