Figure 2. Development of a locally applicable Pluronic gel that ensures extended H₂S release both ex‐vivo and in‐vivo.

A through C, All samples were incubated with 0.25 µmol/L SF_7‐AM in a 96‐well plate in triplicates for 30 minutes at 37°C and then imaged. Resulting fluorescent intensity was corrected for background signal with a PBS+SF₇‐AM control. A, NaHS (1 mmol/L), GYY (1 mmol/L, 250 µmol/L), and PBS dissolved in equal volume PBS, H₂S release was measured at regular intervals. B, Repeated measurement of same wells in 96‐wells plate to assess release rate of respective H₂S releasing compounds. C, H₂S‐drugs dissolved in equal volume of 40% Pluronic gel and overlaid with equal volume PBS. At regular intervals H₂S release in PBS supernatant was measured. GYY indicates hydrogen sulfide prodrug; H₂S, hydrogen sulfide; NaHS, sodium hydrosulfide; and SF₇‐AM, fluorescent probe binding free hydrogen sulfide.