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. 2020 Nov 20;9(23):e016387. doi: 10.1161/JAHA.120.016387

Figure 1. Effect of streptozotocin (STZ) treatment on plasma glucose and urinary excretion of albumin and plasminogen in wild‐type (WT) and plasminogen deficient (Plg−/−) mice.

Figure 1

A, Diabetic wildtypes (WT STZ, n=10) and Plg−/− (KO STZ, n=7) mice displayed a progressive increase in plasma glucose over 4 weeks, whereas nondiabetic controls (WT VEH, n=14; KO VEH, n=4) continued to be at the same level as the baseline. B, The 24‐four hour urine albumin excretion was measured 3 weeks after streptozotocin treatment. Urine albumin was significantly elevated by streptozotocin treatment compared with vehicle in both genotypes (WT STZ, n=8, and KO STZ, n=5; WT VEH, n=10, and KO VEH, n=6). Albuminuria was significantly higher in KO VEH compared with WT VEH. **P<0.01 ****P<0.0001, Mann–Whitney. C, Immunoblotting demonstrated plasminogen in plasma and 24‐hour urine from diabetic WT (WT STZ) mice as a band migrating at ≈90 to 100 kDa, which was absent in urine from Plg−/− (KO) mice. In the bottom blot, the proteins migrated a little slower at the right margin, which caused the apparent different molecular weight.