Figure 5.

Receptor-mediated transcytosis is one of the most common techniques to increase the delivery of large molecules, nanoparticles, and brain-impermeant drugs to brain tumors. Cargo bound to endothelial-membrane-bound receptors is pulled into endothelial cells (ECs) and sorted in the early endosome. Bivalent-binding and high-affinity cargo-receptor complexes are often trafficked to the lysosome for degradation, whereas cargoes bound with lower affinity are more likely to be trafficked for transport across the cell. The cargo is then released on the abluminal side of the endothelium, and the receptor may be recycled back to the luminal membrane.