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. 2020 Dec 13;13(24):5690. doi: 10.3390/ma13245690

Figure 6.

Figure 6

Irritation evaluation using the hen’s egg test-chorioallantoic membrane (HET-CAM) assay. (A) Stereomicroscopic images of the CAM vasculature before treatment (T0) and 5 min after treatment with extraction medium of test orthodontic implants P1, P2, and P3, next to sodium lauryl sulfate (SLS) 0.5% (positive control) and phosphate buffered saline (PBS) (negative control); scale bars 500 µm. (B) Irritation scores of the test orthodontic implants P1, P2, and P3. The experiment was performed in triplicate.