Table 2.
Antibodies (AB), immunohistochemistry (IHC) protocol and resulting staining patterns.
| Antigen | Primary AB (Cat.-No., Clone, Species, Dilution, Incubation) |
Primary AB Supplier | Fluorochrome (Cat.-No.) | Fluorochrome Supplier |
Staining Pattern |
|---|---|---|---|---|---|
| CD73/NT5E | 13160, D7F9A, rabbit (mono), 1:50, for 1 h at 28−30 °C | Cell Signaling Technology, Danvers, MA (USA) | TSA Plus Cyanine 5 (NEL745001KT) |
PerkinElmer, Waltham, MA, USA | See main text |
| GLUT-1 | GTX62480, EPR3915, rabbit (mono), 1:500, overnight at 4 °C |
GeneTex, Irvine, CA (USA) |
Alexa Fluor 488 Tyramide (B40953) |
Thermo Fisher Scientific, Waltham, MA, USA | Membranous |
| CD8 | M7103, CD8/144B, mouse (mono), 1:50, for 1 h at 28−30°C | DAKO, Glostrup, Denmark A/S (DK) | TSA Plus Cyanine 3 (NEL744001KT) | PerkinElmer, Waltham, MA, USA | Membranous |
| Na+-K+- ATPase | ab76020, EP1845Y, rabbit (mono), 1:100, for 1 h at 28–30 °C | Abcam, Cambridge (UK) | Alexa Fluor 647 Tyramide (T20951) | Thermo Fisher Scientific, Waltham, MA, USA | Membranous |
| CD34 | ab81289, EPR373Y, rabbit (mono), 1:100, overnight at 4 °C |
Abcam, Cambridge (UK) | Alexa Fluor 488 Tyramide (B40953) |
Thermo Fisher Scientific, Waltham, MA, USA | Membranous (endothelial cells) |
| CD45 | M0701, 2B11+PD7/26, mouse (mono), 1:50, for 1 h at 28−30 °C | DAKO, Glostrup, Denmark A/S (DK) | TSA Plus Cyanine 3 (NEL744001KT) | PerkinElmer, Waltham, MA, USA | Membranous |
| CD39/ENTP1 | NBP2-45447, OTI2B10, mouse (mono), 1:100, for 1 h at 28–30 °C | Novus Biologicals, Littleton, CO (USA) |
TSA Plus Cyanine 5 (NEL745001KT) |
PerkinElmer, Waltham, MA, USA | See main text |