Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 13;8(12):602. doi: 10.3390/biomedicines8120602

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Transgenic adenocarcinoma of the mouse prostate (TRAMP)-C1-derived tumors show tumor-type-specific anti-inflammatory environments. (A) The outline of analyzing the tumor microenvironment. Following a subcutaneous injection, tumor tissues (Tumor#1 (T#1) and T#2) were analyzed for transcription expressions of selected genes (mRNA) or processed to single-cell suspensions without red blood cells to detect the secreted factors in the conditioned medium (cytokine/chemokine secretions). (B) Comparison of mRNA levels of proinflammatory chemokines (Cxcl1 and Ccl2) between the LLC1 cell line and its derived tumor samples (T#1 and T#2). (C) Comparison of the mRNA levels of the proinflammatory chemokines (Cxcl1 and Ccl2) between the TRAMP-C1 cell line and its derived tumor samples (T#1 and T#2). RT-qPCR measurements were derived from three technical replicates, and the results are presented as the mean ± SD. Student’s t-test. **** p < 0.0001. (D) Comparison of the cytokine/chemokine profiles of the LLC1 cell line and its derived tumors (T#1 and T#2). Differentially secreted factors are labeled with black arrows. (E) Comparison of the cytokine/chemokine profiles of the TRAMP-C1 cell line and its derived tumors (T#1 and T#2). Secretion of the interleukin-1 receptor antagonist (IL1RN) was increased in the tumors (white arrows).

Transgenic adenocarcinoma of the mouse prostate (TRAMP)-C1-derived tumors show tumor-type-specific anti-inflammatory environments. (A) The outline of analyzing the tumor microenvironment. Following a subcutaneous injection, tumor tissues (Tumor#1 (T#1) and T#2) were analyzed for transcription expressions of selected genes (mRNA) or processed to single-cell suspensions without red blood cells to detect the secreted factors in the conditioned medium (cytokine/chemokine secretions). (B) Comparison of mRNA levels of proinflammatory chemokines (Cxcl1 and Ccl2) between the LLC1 cell line and its derived tumor samples (T#1 and T#2). (C) Comparison of the mRNA levels of the proinflammatory chemokines (Cxcl1 and Ccl2) between the TRAMP-C1 cell line and its derived tumor samples (T#1 and T#2). RT-qPCR measurements were derived from three technical replicates, and the results are presented as the mean ± SD. Student’s t-test. **** p < 0.0001. (D) Comparison of the cytokine/chemokine profiles of the LLC1 cell line and its derived tumors (T#1 and T#2). Differentially secreted factors are labeled with black arrows. (E) Comparison of the cytokine/chemokine profiles of the TRAMP-C1 cell line and its derived tumors (T#1 and T#2). Secretion of the interleukin-1 receptor antagonist (IL1RN) was increased in the tumors (white arrows).