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. 2020 Dec 13;8(12):602. doi: 10.3390/biomedicines8120602

Figure 2.

Figure 2

Association of interleukin-1 receptor antagonist (IL1RN) with cluster of differentiation 11b (CD11b)-deficient (CD11b−) tumor-infiltrating leukocytes (TILs) in the transgenic adenocarcinoma of the mouse prostate (TRAMP)-C1-derived tumor microenvironment (TME). (A) Outline for analyzing cytokine/chemokine profiles of leukocytes. Single-cell suspensions prepared from the spleen (orange) or tumors were separated by a Ficoll gradient to enrich leukocytes followed by CD11b-positive cell isolation using an immunomagnetic approach. Secreted factors in conditioned media from different populations were analyzed. (B) Characterization of CD11b-negative (−) and -positive (+) populations from splenocytes and TILs. Cell lysates were analyzed by Western blotting. (C) Comparison of cytokine/chemokine profiles of CD11b− and CD11b+ populations from splenocytes. (D) Comparison of cytokine/chemokine profiles of CD11b− and CD11b+ populations from TILs. Selected factors were labeled with white arrows (IL1RN, CXCL1, and CCL2). (E,F) Correlation of the human IL1RN with CCL2 and with CXCL1. mRNA expression z-scores were plotted on a log-scale. Gene expressions from a human prostate adenocarcinoma database, The Cancer Genome Atlas (TCGA) Program, were analyzed on the website cBioPortal for Cancer Genomics.