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. 2020 Dec 13;8(12):602. doi: 10.3390/biomedicines8120602

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Proliferation effects of the secreted factors in conditioned media from cluster of differentiation 11b (CD11b)-deficient tumor-infiltrating leukocytes (TILs/CD11b⁻). (A) Colony-formation assays using conditioned media from CD11b⁻ and CD11b⁺ leukocytes enriched from splenocytes. Conditioned media were collected at different times (Days 2 and 4) and applied to transgenic adenocarcinoma of the mouse prostate (TRAMP)-C1. (B) Similar to panel A, conditioned media were collected from TILs enriched from TRAMP-C1-derived tumors. (C) Comparison of colony numbers in TRAMP-C1 cells incubated with conditioned media derived from either CD11b⁻ or CD11b⁺ leukocytes. Measurements were derived from three replicates, and results are presented as the mean ± SD. Student’s t-test. ** p < 0.01, *** p < 0.001.