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. 2020 Dec 13;8(12):602. doi: 10.3390/biomedicines8120602

Figure 4.

Figure 4

Proliferation effects of the interleukin (IL)-1 receptor antagonist (IL1RN) in transgenic adenocarcinoma of the mouse prostate (TRAMP)-C1 cells. (A) Colony assays using selected chemokines identified in cluster of differentiation 11b (CD11b)-negative (CD11b−) cells collected from TRAMP-C1-derived tumors. TRAMP-C1 cell lines were incubated with an individual chemokine (50 ng/mL). Images were processed by Image J using the ColonyArea plug-in [17]. (B) Quantification of the colony size and staining signal. Proliferation was indicated by the analyzed colony features, such as the occupied sizes (Areas) and staining signals (Intensities), from Panel (A); n = 3. (C) Monitoring of proliferation by a 3D culture approach. Proliferation effects of the IL1RN were indicated by colony numbers calculated from the images; n = 3. (D) Monitoring of DNA synthesis of TRAMP-C1 cells following IL1RN treatment. DNA synthesis was measured by BrdU incorporation. Measurements were derived from three replicates, and the results are presented as the mean ± SD. Student’s t-test. * p < 0.05, ** p < 0.01.