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. 2020 Dec 9;9(12):2643. doi: 10.3390/cells9122643

Figure 8.

Figure 8

Ferroptosis: Cell proliferation of T24par and T24res after 72 h incubation with 10 µM ART alone or plus 20 µM ferrostatin-1 (Fer-1), a ferroptosis inhibitor, in percent (A). Untreated controls were set to 100%. Error bars indicate standard deviation (SD). Significant difference to untreated control: ** p ≤ 0.01, *** p ≤ 0.001. n = 3. Representative Western blot of GPX4 expression in T24 cells after 24 h exposure to 10 µM ART (B). par = parental, res = resistant. Pixel density analysis of the GPX4 expression (C), compared to untreated controls (set to 100%) is illustrated. Each protein analysis was normalized by a total protein staining control. Error bars indicate standard deviation (SD). n = 4. For detailed information regarding the Western blots see Figure S4.