Table 4.

Test of primers used for qRT-PCR expression analysis of apoptosis-related genes of rats.

Target Gene	Forward (F) and Reverse (R) Primers (5′-3′)	GenBank Accession No.	T(a), °C
Caspase-3	F: 5′-CAGAGCTGGACTGCGGTATTGA-3′ R: 5′-AGCATGGCGCAAAGTGACTG-3′	NM_012922	60
Caspase-9	F: 5′-AGCCAGATGCTGTCCCATAC-3′ R:5′-CAGGAGACAAAACCTGGGAA-3′	AF262319	60
Tp53	F: 5′-GTCGGCTCCGACTATACCACTATC-3′ R:5′-CTCTCTTTGCACTCCCTGGGGG-3′	NM_030989	60
GAPDH	F: 5′-GCTGCCTTCTCTTGTGACAAAGT-3′ R: 5′-CTCAGCCTTGACTGTGCCATT-3′	AF106860	60