Figure 3.

Pyranoxanthone 2 (PX2) treatment results in chromosome misalignment phenotype. (a) Immunofluorescence images of an untreated cell in metaphase with all chromosomes aligned at equatorial zone (Top), and a PX2-treated cell, arrested in prometaphase-like state, showing many misaligned chromosomes (Bottom). Microtubules (green) were stained with anti-α-tubulin antibody and DNA (blue) with 4’,6’-diamino-2-fenil-indol (DAPI). Bar, 5 µm. (b) Graphical representation of data shown in (a), in absence (−) or presence (+) of proteasome inhibitor MG-132 with statistical relevance of **** p < 0.0001 by two-way ANOVA with Tukey’s multiple comparisons test.