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. 2020 Dec 10;25(24):5845. doi: 10.3390/molecules25245845

Figure 4.

Figure 4

Pyranoxanthone 2 (PX2) treatment generates loss of stability of kinetochore-microtubule attachments. (a) Representative immunofluorescence images after cold treatment assay, showing several unattached kinetochores (free red spots pointed by the white arrowheads) in cells with PX2 treatment, whereas most kinetochores were attached (Red spots with attached green fibers) in untreated cells. Microtubules (green) were stained with anti-α-tubulin antibody, kinetochores (red) with anti-CREST antibody, and DNA (blue) with DAPI. (b) Quantification of cold-stable microtubules (as percentage of attached kinetochores per cell) after treatment with PX2 with statistical relevance of ** p < 0.01 by unpaired t-test.