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. 2020 Dec 9;21(24):9365. doi: 10.3390/ijms21249365

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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UBAC1 promotes K63-linked ubiquitination of TANK. (A) HEK293 cells were transfected with the indicated expression vectors. A total of 24 h later, cell lysates were immunoprecipitated with anti-HA antibody, separated by SDS-PAGE and transferred onto membranes subsequently probed with anti-ubiquitin. Statistical analysis for the blot shown in provided as supplementary Figure S1. (B) HEK293 cells were co-transfected with HA-tagged ubiquitin mutants and FLAG-tagged UBAC1 and TANK. The number indicates the only lysine residue remaining in the ubiquitin molecule. Immunoprecipitates with anti-TANK antibody were resolved by SDS-PAGE and blotted onto a membrane subsequently probed with anti-HA or anti- ubiquitin (P4D1). Statistical analysis for the blot shown in provided as supplementary Figure S1.