Table 1.
Interaction of CARMA2sh with UBAC1 in the yeast two-hybrid assay. Yeast AH109 was transformed with CARMA2sh fused to the GAL4-DNA binding domain together with the indicated cDNAs fused to GAL4-activating domain. The cDNA encoding for FADD was used as a putative negative control. Interactions were examined by yeast growth on selective media; assays were done for ten independent transformants. Yeast colonies were scored as positive (+) when a growth developed within 36 h; a negative (-) was scored when growth failed to develop within 5 days.
| Protein Fused to GAL4 Domain | Yeast Growth on Selective Media | |
|---|---|---|
| DNA-Binding | Activating | |
| - | UBAC1 | - |
| Vector | UBAC1 | - |
| FADD | UBAC1 | - |
| CARMA2sh | UBAC1 | +++ |
| CARMA2sh | - | - |