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. 2020 Dec 11;21(24):9448. doi: 10.3390/ijms21249448

Figure 4.

Figure 4

Biological activity of (S,S)-3-oxo-C12-ACH: (a) IL-8 secretion of Caco-2/TC7 cells exposed to inflammation and increasing doses of (S,S)-3-oxo-C12-ACH for 18 h. Secreted IL-8 normalized to control DMSO. Points are the mean of several replicates (n ≥ 8) ± SEM. No statistical difference vs. control observed in Tukey’s post-test, yet positive one-way ANOVA (p < 0.0001). (b) IL-6 secretion of RAW264.7 cells exposed to LPS/TNF-α and increasing doses of (S,S)-3-oxo-C12-ACH for 6 h. Secreted IL-6 normalized to control DMSO. Points are the mean of several replicates (n ≥ 8) ± SEM. * p < 0.05, ** p > 0.01, *** p < 0.001 vs. control, one-way ANOVA (p < 0.0001), Tukey’s post-test. (c) Compared bioluminescence in reporter strain E. coli pSB1075 with increasing doses of 3-oxo-C12-HSL (1) and (S,S)-3-oxo-C12-ACH (30). Points are the mean value of several replicates (n ≥ 6) ± SEM.