Table 2.
Intra- and inter-day precision and accuracy values for SCs and their metabolites in OF by GC-MS and UHPLC-HRMS.
| GC-MS | |||||||||
| Analytes |
Intra-Day Precision
(CV%) |
Inter-Day Precision
(CV%) |
Accuracy
(% Error) |
||||||
| QC Samples a | |||||||||
| Low | Medium | High | Low | Medium | High | Low | Medium | High | |
| JWH-122 | 4.8 | 7.3 | 9.4 | 9.7 | 8.7 | 6.9 | 8.7 | 8.7 | 7.3 |
| JWH-210 | 3.2 | 9.1 | 5.3 | 9.9 | 5.3 | 7.2 | 9.9 | 10.2 | 10.1 |
| UR-144 | 5.7 | 9.2 | 8.3 | 10.1 | 12.3 | 11.2 | 9.1 | 8.5 | 9.9 |
| UHPLC-HRMS | |||||||||
| Analytes |
Intra-Day Precision
(CV%) |
Inter-Day Precision
(CV%) |
Accuracy
(% Error) |
||||||
| QC Samples a | |||||||||
| Low | Medium | High | Low | Medium | High | Low | Medium | High | |
| JWH-122 | 2.2 | 4.2 | 8.6 | 11.4 | 5.7 | 5.9 | 13.7 | 9.9 | 7.7 |
| JWH-122 N-(4-OH) | 4.1 | 6.9 | 3.1 | 10.1 | 8.3 | 7.4 | 10.6 | 9.6 | 3.7 |
| JWH-122 N-(5-OH) | 7.6 | 15.4 | 12.8 | 12.0 | 15.4 | 11.9 | 8.7 | 7.5 | 13.5 |
| JWH-210 | 6.2 | 5.2 | 8.4 | 15.6 | 5.4 | 6.9 | 8.5 | 9.8 | 4.2 |
| JWH-210 N-(4-OH) | 4.8 | 7.1 | 6.2 | 12.6 | 7.1 | 5.2 | 11.4 | 8.7 | 10.3 |
| JWH-210 N-(5-OH) | 15.1 | 7.7 | 8.5 | 17.4 | 11.2 | 8.4 | 8.0 | 10.3 | 10.4 |
| UR-144 | 7.6 | 15.4 | 12.8 | 12.0 | 15.4 | 11.9 | 8.7 | 7.5 | 10.8 |
| UR-144 N-(5-OH) | 4.1 | 6.9 | 3.8 | 10.1 | 8.0 | 7.0 | 10.6 | 9.6 | 3.7 |
a Mean of five replicates (n = 15) along five subsequent working days.