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. 2020 Dec 8;10(12):1646. doi: 10.3390/biom10121646

Figure 2.

Figure 2

Knockdown (KD) of lncRNA-DANCR expression in NSCLC cells inhibits migration and clonogenic growth. (A) Fold change of DANCR expression in NSCLC cell lines A549, H358, H1975, and H1755 transiently transfected with two different DANCR siRNAs (n = 3, t test, ** p < 0.01, **** p < 0.0001); (B) effect of DANCR KD on cell growth of A549 and H1755 cells using Aqueous Cell Titer 96 reagent (n = 3, 2-way ANOVA, ** p < 0.01, *** p < 0.001); (C) cell impedance assay using xCELLigence RTCA SP system on A549 and H1755 DANCR KD cells compared to scramble control (n = 3); (D) colony-formation assay on A549 and H1755 two weeks after seeding (n = 3, t test, **** p < 0.0001).