Table 9.
Analytical methods for gyromitrine detection.
| Ref. | Matrix | Separation | Detection | Qualitative/Quantitative | LOD | LOQ | Linearity | Extraction Recovery | Additional Analytical Information |
|---|---|---|---|---|---|---|---|---|---|
| [206] | Viscera | TLC | UV (254–277 nm) IR (NC) |
Qualitative and quantitative | NC | NC | 0.1–0.5 g/L | NC | - |
| [219] | Mice gastric content | GC | UV and IR | Quantitative | NC | NC | NC | NC | Column: (2 mm × 2 mm) Chromosorb 103; T column: 160 °C; Helium flow rate: 20 mL/min; RT: GYRO: 17 min, MFH: 15.7 min |
| [220] | Mushrooms | GC | MS | Quantitative | NC | NC | NC | NC | Column: 50 m FFAP |
| [221] | Mice peritoneal fluids | GC | MS | Quantitative (MH) | NC | NC | NC | NC | - |
| [222] | Mushrooms | TLC | Spectrofluorimetry (λexcitation = 340 nm; λemission = 610 nm) |
Quantitative | NC | NC | 0.43–2.17 ng | NC | - |
| [223] | Mushrooms | GC | FID | Quantitative | NC | NC | NC | 30–74% GYRO 96–124% MH |
Column: (25 mm × 0.31 mm) SE-54; Helium flow rate: 1 mL/min; RT: 7.3 min |
| [224] | Mushrooms | GC | EI-MS (Full-scan 35–650 m/z) |
Quantitative | MH: 12 µg/L = 0.3 µg/g of gyromitrin | NC | NC–1.2 mg/L | 36–55% | Column: (30 mm × 0.25 mm) 0.25 µm HP5-MS |
| [56] | Standard solution | - | PSI-HR-MS/MS (101.0713 to 73.0764 m/z) |
Qualitative | NA | NA | NA | NA | - |
NA: not applicable; LOD: limit of detection; LOQ: limit of quantification; NC: not communicated; GYRO: gyromitrin; MFH: N-methyl-N-formylhydrazine; MH: methylhydrazine.