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. 2020 Dec 11;10(12):2380. doi: 10.3390/ani10122380

Table 7.

Effect of linseed oil supplementation on ruminal Protozoa and Methanogen populations 1.

Items Treatment 2 Measurement Period 3 p-Value
SA LO SEM MP1 MP2 SEM Treatment MP Treatment x MP
Methanogens 1.9877 1.7332 0.2744 2.1733 1.5477 0.2905 0.311 0.18 0.215
Protozoa 6.3317 6.7198 1.2730 3.2446 9.8068 1.1562 0.806 0.028 0.601
Methanosphaera stadtmanae 4 0.0184 0.0061 0.00027 0.0100 0.00560 0.00029 0.19 0.21 0.169
Methanobrevibacter smithii 4 0.2248 0.1169 0.00439 0.1824 0.1497 0.0062 0.12 0.67 0.18
Methanobrevibacter ruminantium 4 0.1796 0.0879 0.00371 0.1776 0.0920 0.0038 0.011 0.326 0.269

1 Methanogens and Protozoa measured as relative abundance = 2−(Ct target-Ct total bacteria) × 102; 2 SA = supplementary concentrate containing 16% FW stearic acid; LO = supplementary concentrate containing 16% FW linseed oil; 3 MP = measurement period, MP 1 corresponds to 17 to 22 d post treatment introduction and MP 2 corresponds to 44 to 49 d after treatment introduction; 4 Methanogen spp. Measured as a proportion of total estimated rumen methanogen 16S rDNA, relative quantification = 2−(Ct target-Ct total methanogens).