Table 2.

Analysis of variance (ANOVA) for the response surface quadratic model for FOS production.

	Enzyme Activity		1-Kestose		Nystose		1F-β-Fructofuranosylnystose		Total FOS
	F Value	p Value	F Value	p Value	F Value	p Value	F Value	p Value	F Value	p Value
Model	24.57	0.0002	8.64	0.0048	75.78	<0.0001	54.54	<0.0001	42.45	<0.0001
X1 *	2.10	0.1904	0.985	0.354	2.13	0.168	3.18	0.0979	1.58	0.248
X2 **	2.18	0.1832	2.87	0.134	0.573	0.462	0.545	0.473	3.78	0.0931
X3 ***	191.53	<0.0001	27.92	0.0011	224.63	<0.0001	159.89	<0.0001	310.96	<0.0001
X 1 X 2	1.18	0.3135	2.03	0.197					1.91	0.210
X 1 X 3	0.225	0.6494	0.173	0.690					1.76	0.226
X 2 X 3	0.304	0.5984	0.0128	0.913					0.247	0.634
X 1 2	0.0859	0.7780	0.230	0.646					1.66	0.239
X 2 2	0.0307	0.8660	0.694	0.432					0.00004	0.995
X 3 2	23.34	0.0019	43.55	0.0003					58.68	0.0001
Lack of Fit	5.61	0.0645	6.06	0.0572	1.27	0.438	0.981	0.553	4.34	0.0951

* X₁, ultrasonication time (min); ** X₂, Microbubble time (min); *** X₃, Reaction time (h).