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. Author manuscript; available in PMC: 2020 Dec 26.
Published in final edited form as: Cell Rep. 2020 Dec 8;33(10):108486. doi: 10.1016/j.celrep.2020.108486

Figure 6. A Conserved MCU Gating Mechanism.

Figure 6.

(A) Ca2+ transport mediated by various MCU homologs. Corresponding residues of Y281 and Y289 are Y212 and F220 in D. discoideum (Dd) MCU, Y214 and P222 in A. thaliana (At) MCU, and Y280 and Y288 in D. melanogaster (Dm) MCU. All of the mutants were expressed in ME-KO cells. Dm MCU was co-expressed with Dm EMRE. Dd MCU is poorly inhibited by Ru360.

(B) coIP experiments comparing complex formation of various hMCU mutants with WT hEMRE. 1D4-tagged MCU was used to pull down EMRE co-expressed in ME-KO cells.

Data are presented as means ± SEMs.

See also Figure S4.