KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
EMRE	Santa Cruz	Cat# 86337; RRID: AB_2250685
β-actin	Santa Cruz	Cat#69879; RRID: AB_1119529
1D4	Home made	(Tsai et al., 2016)
C8	Home made	(Tsai et al., 2016)
IRDye 680RD goat anti-rabbit	Li-Cor	Cat#926-68071; RRID: AB_10956166
IRDye 680RD goat anti-mouse	Li-Cor	#925-68070; RRID: AB_2651128
Biological Samples
Extracted Xenopus laevis ovaries	Nasco	LM00935
Chemicals, Peptides, and Recombinant Proteins
Ru360	Home made	(Tsai et al., 2016)
Digitonin	Sigma-Aldrich	D141
n-Dodecyl-β-D-Maltopyranoside, anagrade	Anatrace	D310
Calcium green 5N	ThermoFisher	C3737
CNBr-activated Sepharose 4B	GE Healthcare	17043001
ETH129	Sigma-Aldrich	21193
Methoxypolyethylene glycol maleimide (5,000 Da)	Sigma-Aldrich	63187
Experimental Models: Cell Lines
HEK293T	ATCC	CRL-11268
MCU-KO HEK293T	Home made	(Tsai et al., 2016)
EMRE-KO HEK293T	Home made	(Tsai et al., 2016)
MCU/EMRE-KO HEK293T	Home made	(Tsai et al., 2016)
Recombinant DNA
Various MCU or EMRE mutants in pcDNA3 (+)	Home made	This work
Software and Algorithms
Clustal Omega	Online Server	https://www.ebi.ac.uk/Tools/msa/clustalo/
Excel (version 16)	Microsoft	https://www.microsoft.com/en-us/microsoft-365/excel
Igor Pro (version 8)	WaveMetrics	https://www.wavemetrics.com/products/igorpro
ImageStudio (version 5.2)	Li-Cor	https://www.licor.com/bio/image-studio/