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. 2020 Dec 12;25(24):5881. doi: 10.3390/molecules25245881

Figure 1.

Figure 1

Cytotoxic and anti-proliferative effect of LOB3 in cancer cells. (A) Chemical structure of LOB3. (B) C6 cells were treated with the indicated doses of LOB3 for 48 h, and cell viability was determined by a conventional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (C) C6 cells were treated with LOB3 (20 μM) for the indicated time, and viable cell numbers were determined by a conventional MTT assay. (D,E) C6 cells were treated with the indicated doses of LOB3 for the indicated time, and cell numbers and shapes were observed under a light microscope. Photos of the cells were taken by a digital camera (D) and numbers of cells were counted by a cell counter (E). (F–H) U251, MDA-MD-231, and peritoneal macrophage cells were treated with the indicated doses of LOB3 for 48 h, and cell viability was determined by a conventional MTT assay. The data (B,C,E,F–H) are expressed as the means ± standard error of the mean (SEM) of three independent experiments. Statistical significance was analyzed by the Mann-Whitney U test. ** p < 0.01 compared to the vehicle-treated controls.