Figure 5.

gpx3 suppression activates apoptosis and inhibits cell proliferation in gpx3 morphants. (A) The expression and number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells were higher in gpx3 knockdown embryos compared to control embryos. (B) Relative TUNEL expression, indicating a significant increase in apoptotic cells in gpx3 morphants. ** p < 0.001. (C) gpx3 loss resulted in reduced phospho-histone 3 (PH3)-positive cells in the tail region of gpx3 knockdown embryos. PH3-positive cells are indicated by the color purple, whereas β-gal staining is indicated by the color blue. (D) The relative PH3 expression showed cell proliferation was significantly reduced by gpx3 knockdown. * p < 0.05.