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. 2020 Nov 27;2(1):vdaa163. doi: 10.1093/noajnl/vdaa163

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© The Author(s) 2020. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — PLAGL1 binds to the promoter region for CD109 to positively regulate its transcriptional activity. (A) Chromosome Immunoprecipitation (ChIP)-qPCR assay showing PLAGL1 binding to the promoter region for the CD109 gene in g1051E and g101027E spheres. H3K9Ac is used as a positive control. (B) Western blotting of CD109 and PLAGL1 in g1051E and g101027E spheres after transducing with overexpression vector, shRNA targeting PLAGL1 (sh#1 or sh#2), or a nontargeting control (Ctrl).