Table 4.
Methylglyoxal adducts detected in the reaction mixture of standard compounds and water infusion of G. officinalis after 1 h incubation with MGO. Quercetin and metformin hydrochloride were used as a positive control.
| Compound | Source | Peak | Mono-MGO Adduct (m/z) | di-MGO Adduct (m/z) |
|---|---|---|---|---|
| Chlorogenic acid | S | - | n.d. | n.d. |
| Rutin | S | a | 681.1682 [M − H]− | 753.1892 [M − H]− |
| b | 681.1695 [M − H]− | 753.1890 [M − H]− | ||
| c | 681.1683 [M − H]− | 753.1885 [M − H]− | ||
| Inf | a | 681.1684 [M − H]− | n.d. | |
| Quercetin | S | a | 373.0569 [M − H]− | 445.0779 [M − H]− |
| b | 373.0564 [M − H]− | n.d. | ||
| Galegine sulfate | S | a | 200.1367 [M + H]+ | n.d. |
| b | 200.1364 [M + H]+ | n.d. | ||
| c | 200.1364 [M + H]+ | n.d. | ||
| Galegine | Inf | a | 200.1397 [M + H]+ | n.d. |
| b | 200.1386 [M + H]+ | n.d. | ||
| c | 200.1388 [M + H]+ | n.d. | ||
| Hydroxygalegine | Inf | a | 216.1334 [M + H]+ | n.d. |
| Metformin hydrochloride | S | a | 202.1282 [M + H]+ | n.d. |
S: standard compound; Inf, water infusion; n.d.: not detected.