Table 4.

Pairwise comparison of morula groups (I–IV) with different developmental potential according to the fold change in the detection level of sncRNAs in spent culture medium assessed by qPCR.

sncRNA	Sample Group	Me 1	Log2(Me)	Log2(Q1)	Log2(Q3)	p-Value
						I vs. III + IV	I vs. II	II vs. III + IV
hsa_piR_011291	I	3.3173	1.73	0.38	2.38	0.023651
	III + IV	1.6245	0.7	−0.31	1.14
	II	1.3104	0.39	−0.4	0.9
hsa_piR_019122	I	13.0864	3.71	2.56	4.6	0.043099
	III + IV	6.9163	2.79	2.11	3.31
	II	5.5022	2.46	2.26	2.87
hsa_piR_001311	I	4.1699	2.06	0.98	2.73	0.003247
	III + IV	0.9862	−0.02	−1.86	1.09
	II	1.5801	0.66	−0.79	2.09
hsa_piR_015026	I	2.1435	1.1	−0.95	2.67	0.030825
	III + IV	0.5987	−0.74	−1.47	−0.08
	II	0.4033	−1.31	−1.56	−1.01
hsa_piR_015462	I	7.9447	2.99	0.65	3.67	0.003247	0.015984
	III + IV	1.0210	0.03	−0.58	0.62
	II	0.7022	−0.51	−1.14	−0.04
hsa_piR_016735	I	4.1699	2.06	1.41	2.66	0.013416
	III + IV	1.8404	0.88	0.3	1.58
	II	1.6358	0.71	−0.54	0.81
hsa_piR_019675	I	28.2465	4.82	2.58	7.2	p < 0.001	p < 0.001	0.031124
	III + IV	1.2834	0.36	−0.33	1.01
	II	0.7900	−0.34	−0.67	0.01
hsa_piR_020381	I	2.8089	1.49	1.14	2.88	0.003247	0.002997
	III + IV	1.3104	0.39	0.04	1.26
	II	1.0792	0.11	−0.12	0.35
hsa_piR_020485	I	1.1567	0.21	−2.27	1.37	0.001523
	III + IV	0.0171	−5.87	−13.71	−2.95
	II	0.0349	−4.84	−6.64	−1.78
hsa_piR_004880	I	4.2871	2.1	0.65	2.34	p < 0.001	0.004745
	III + IV	0.9266	−0.11	−0.36	0.09
	II	0.9659	−0.05	−0.21	0.04
hsa_piR_000807	I	0.1975	−2.34	−5.62	0.53	0.012145
	III + IV	0.0013	−9.55	−13.58	−2.43
	II	0.0011	−9.89	−12.96	−3.85
hsa-let-7b-5p	I	230.7201	7.85	4.59	9.13	0.00462
	III + IV	13.6422	3.77	1.58	5.26
	II	6.6346	2.73	−5.2	6.07
hsa-let-7i-5p	I	5.2416	2.39	2.15	2.95	0.001976		0.006596
	III + IV	2.1735	1.12	0.79	1.49
	II	3.5554	1.83	1.72	2.58

¹ Data are presented as medians (Me) of the fold change in the detection level of sncRNA in the spent culture medium relative to the reference medium without any embryo and quartiles Q1 and Q3.