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. 2020 Dec 22;12:13137–13148. doi: 10.2147/CMAR.S274447

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© 2020 Chen et al.

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Knockdown of miR-519d-3p inverted the functional effects of BLACAT1 deletion, and decreased the OXA sensitivity of CRC cells in vitro. MiR-NC, miR-519d-3p, si-BLACAT1#1+anti-NC or si-BLACAT1#1+anti-miR-519d-3p was separately transfected into CRC cells. (A) QRT-PCR was used to confirm the level of miR-519d-3p in each group. (B–C) The cell viability was analyzed by MTT assay at stated times (24 h, 48 h, 72 h) in HCT116 and HT29 cells. (D) Flow cytometry was conducted to analyze the cell apoptosis rate. (E–F) Cell migration and invasion were evaluated by transwell assay. (G–I) The expression of Cleaved-cas3 and MMP-9 was measured by Western blot. HCT116/OXA and HT29/OXA cells were given similar administration above, (J) the IC50 of OXA was determined by MTT assay, and (K) the cell apoptotic rate was explored by flow cytometry analysis. *P<0.05.

Abbreviations: BLACAT1, bladder cancer-associated transcript 1; miR-519d-3p, microRNA-519d-3p; CRC, colorectal cancer; OXA, oxaliplatin; IC50, half maximal inhibitory concentration.