Figure 2.

Stemness markers of monolayers and spheroids from RT112 and 5637 cells. (a–c) Median fluorescence intensity of Aldefluor^TM (a), CD44 (b) and CD133 (c) by flow cytometry analysis on RT112 and 5637 cells grown as monolayers. Results are the mean of two (a) and three (b,c) experimental replicates. Statistical test: t-test, * for p < 0.05. (d) Representative images from confocal immunofluorescence (IF) microscopy of RT112 and 5637 cells using SOX2 Antibody (red) and Hoechst 33342 (blue) for nuclei. Cells were grown as monolayer or spheroids on different (Tissue culture-treated, Not-treated or Cell repellent) supports, before being seeded in adherent condition on chamber slides for IF.