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. 2020 Dec 11;9(12):2669. doi: 10.3390/cells9122669

Figure 7.

Figure 7

Effect of pharmacological treatment on proliferation and migratory capacity of RT112 and 5637 cells grown as adherent cells. (a) Representative images of brightfield (T0) and Hoechst 33342 stained nuclei acquired with Operetta CLS™ of adherent cells after 72 h-treatment with increasing concentration of 2-Deoxy-D-glucose (2-DG) (0, 0.5, 5, 15 mM) and metformin (0, 1, 5, 10, 20 mM). (b) Fold change relative to the control condition of nuclei count of cells treated for 72 h grown with 2-Deoxy-D-glucose (2-DG) and metformin treatment. Results are the mean of three experimental replicates. (c,d) Percentage of wound coverage in wound healing assay after 12 h-treatment condition of increasing concentration of 2-Deoxy-D-glucose (2-DG) (0, 0.5, 5, 15 mM) and metformin (0, 1, 5, 10 mM) of RT112 cells (c) and 5637 cells Results are the mean of two experimental replicates, except for 15 mM 2-DG and 10 mM metformin conditions performed in single replicate. Statistical test: t-test, * for p < 0.05; *** for p < 0.001.