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. 2020 Dec 11;21(24):9442. doi: 10.3390/ijms21249442

Figure 1.

Figure 1

Deletion of the miR-371/372/373 cluster and the miR-371/372/373 promoter in SAS cell using clustered, regularly interspaced, short palindromic repeats (CRISPR)/Cas9 system. (A) Schematic diagram of the approach used. Green box, promoter; Red box, miR-371/372/373 cluster. Scissors and triangles indicate the predicted S1–S3 cleavage sites. F, location of the forward primers; R, location of the reverse primers. (B,C) Deletion of the miR-371/372/373 cluster and the miR-371/372/373 promoter, respectively. Lt, sorting of GFP+ cells being blocked. Middle, electrophoretic gel illustrating the amplicons generated by the different combinations of primers. Red color labels the certified homozygous deletion after repeat examination. Rt, sequencing results. The truncations of the gene segments in the c60 and p49 subclones are shown.