Table 1.
No. | Amino Acid | Retention Time | Selected Ions 1 (m/z) | Amount (ng/mg, Mean ± SD) | Normalized Value 2 | |||
---|---|---|---|---|---|---|---|---|
L. barbarum | L. chinense | |||||||
1 | Alanine | 5.17 | 190 | 218 | 260 | 2187.6 ± 67.8 | 565.2 ± 13.6 | 0.26 |
2 | Glycine | 5.40 | 176 | 204 | 246 | 36.5 ± 1.1 | 132.9 ± 1.0 | 3.64 |
3 | α-Aminobutyric acid | 6.06 | 130 | 204 | 232 | 12.9 ± 0.3 | 29.2 ± 0.6 | 2.27 |
4 | Valine | 6.51 | 144 | 218 | 246 | 290.3 ± 10.4 | 412.8 ± 15.2 | 1.42 |
5 | Leucine | 7.37 | 158 | 232 | 260 | 234.1 ± 8.0 | 683.4 ± 7.2 | 2.92 |
6 | Isoleucine | 7.62 | 158 | 232 | 260 | 55.7 ± 1.5 | 400.7 ± 5.7 | 7.19 |
7 | Proline | 8.09 | 142 | 216 | 244 | 9798.5 ± 242.4 | 2931.3 ± 106.0 | 0.30 |
8 | γ-Aminobutyric acid | 8.12 | 186 | 201 | 346 | 121.4 ± 4.2 | 1108.0 ± 31.3 | 9.13 |
9 | Pipecolic acid | 8.81 | 156 | 230 | 258 | 61.0 ± 1.7 | 97.6 ± 5.0 | 1.60 |
10 | Pyroglutamic acid | 10.25 | 272 | 300 | 342 | 653.7 ± 20.6 | 240.0 ± 8.7 | 0.37 |
11 | Serine | 11.03 | 216 | 302 | 348 | 514.0 ± 59.5 | 530.8 ± 42.9 | 1.03 |
12 | Threonine | 11.12 | 230 | 248 | 261 | 159.6 ± 33.2 | 146.2 ± 17.7 | 0.92 |
13 | Phenylalanine | 12.17 | 195 | 205 | 294 | 39.1 ± 1.7 | 440.7 ± 17.9 | 11.28 |
14 | Cysteine | 12.58 | 216 | 276 | 322 | 157.8 ± 0.5 | 158.7 ± 0.3 | 1.01 |
15 | Aspartic acid | 12.81 | 287 | 330 | 287 | 330 | 1079.9 ± 24.0 | 0.85 |
16 | N-Methyl-DL-aspartic acid | 13.01 | 244 | 287 | 390 | 40.5 ± 1.7 | 23.1 ± 0.5 | 0.57 |
17 | Homocysteine | 13.85 | 234 | 290 | 336 | 81.2 ± 7.6 | 56.5 ± 0.3 | 0.70 |
18 | Glutamic acid | 14.18 | 288 | 344 | 390 | 346.2 ± 15.3 | 559.5 ± 31.9 | 1.62 |
19 | Asparagine | 14.47 | 286 | 329 | 375 | 3460.4 ± 146.9 | 15211.9 ± 1141.6 | 4.40 |
20 | α-Aminoadipic acid | 15.36 | 302 | 358 | 404 | 33.6 ± 0.1 | 39.4 ± 0.7 | 1.17 |
21 | Glutamine | 15.71 | 287 | 343 | 389 | 7462.7 ± 588.4 | 1468.8 ± 119.1 | 0.20 |
22 | Lysine | 15.85 | 156 | 301 | 347 | 185.9 ± 6.1 | 897.2 ± 57.3 | 4.83 |
23 | Histidine | 16.35 | 254 | 267 | 356 | 565.5 ± 9.6 | 1441.7 ± 238.2 | 2.55 |
24 | Tryptophan | 18.35 | 130 | 244 | 333 | 156.8 ± 3.9 | 520.5 ± 118.1 | 3.32 |
1 The bolded ions were used for quantification. 2 Normalized value = amino acid amount of L. chinense/amino acid amount of L. barbarum.