Table 1.
Overview of revised publications related to adipose tissue EVs protein cargo characterization. Reference, method of EV isolation, type of protein analysis, type of cell/tissue or circulating analysis, species, and propose EVs markers are shown. dUC: differential ultracentrifugation; SDG: Sucrose density grandient; UF: ultrafiltration; TEIR: total exosome isolation reagent; LC-MS/MS: liquid chromatography coupled to mass spectrometry; SWATH: sequential window acquisition of all theoretical mass spectra; ELISA: enzyme-linked immunosorbent assay; MV: microvesicles; EXO: exosomes; CRP: C-reative protein; ICAM-1: intercellular adhesion molecule 1; DDP-4: dipeptidyl peptidase-4; GDM: gestational diabetes mellitus; MSCs: mesenchymal stem cells; VAT: visceral adipose tissue; SAT: subcutaneous adipose tissue.
| Ref | Isolation Method | Type of Protein Analysis | Cell/Tissue Type/Circulating | Species | EVs Markers |
|---|---|---|---|---|---|
| Cultured adipocytes | |||||
| Kranendonk et al., 2014 [22] | dUC | Immunoblot + multiplex immunoassay | Simpson Golabi Behmel Syndrome (SGBS) adipocytes cell line | Human | FABP4, adiponectin, TNF-α, MCSF, RBP4, MIF |
| Hartwing et al., 2019 [42] | UF + dUC | NanoLC-MS/MS | Human primary adipocytes from lean to moderate overweight woman | Human | Adiponectin, FABP4, metalloproteases |
| Lazar et al., 2016 [31] | SDG | NanoLC-MS/MS | 3T3-F442A adipocyte cell line | Mouse | ECHA, HCDH (FAO INVOLVED) |
| Durcin et al., 2017 [43] | dUC | NanoLC-MS/MS | 3T3-L1 adipocyte cell line | Mouse | L-Evs: FABP4, 14-3-3, Annexin A2, endoplasmin, actinin-4 sEVs: MVP, FAS, adiponectin |
| Camino et al., 2020 [15] | dUC | Nano-LC/MS-MS + SWATH | C3H10T1/2 adipocyte cell line: models of IR and lipid hypertrophy | Mouse | Ceruloplasmin, mimecan, perilipin 1, TFGBI |
| Lee et al., 2015 [44] | dUC | NanoLC-MS/MS+ label-free | Obese diabetic and obese nondiabetic adipocytes of Otsuka Long-Evans Tokusima Fatty (OLETF) primary cell culture | Rat | Caveolin, LPL, AQ7 |
| Cultured adipose-derived mesenchymal stem cells | |||||
| Eirin et al., 2016 [45] | dUC | LC-MS/MS | MSCs from abdominal fat | Porcine | C2, VEGF, vWF (vonWillebrand factor) |
| Xing et al., 2020 [46] | dUC | LC-MS/MS | Adipose-derived mesenchymal stem cells (ADSCs) | Mouse | Tissue repair |
| Adipose tissue explants | |||||
| Kranendonk et al., 2014 [22] | dUC | Adipokine profile array | Subjects undergoing surgery for aneurysmatic aortic disease (obese/overweight) | Human | MCP-1, IL-6, and MIF in omental AT vs. subc |
| Jayabalan et al., 2019 [47] | dUC | LC-MS/MS(SWATH) | Omental adipose tissue from woman with GDM after baby delivery | Human | Proteins related to glucose metabolism |
| Camino et al., 2020 [48] | dUC | NanoLC-MS/MS (SWATH) | Visceral and Subcutaneous whole AT explants from obese | Human | VAT: TGFBI, CAVN1, CD14, mimecan, thrombospondin-1, FABP-4,AHNAK/↓Syntenin 1 |
| Zhang Y et al., 2020 [49] | TEIR | LC-MS/MS | Inguinal fat pads obese Sprague-Dawley rats | Rat | NPM3, DAD1and STEAP3 |
| Circulating EVs | |||||
| Kranendonk et al., 2014 [50] | ExoQuick | Multiplex immunoassay | Circulating:plasma patients with vascular disease | Human | Cystatin C positive related to obesity metabolic complications/CD14 negative related |
| Kobayashi et al., 2018 [51] | qEV column | Immunoblot | Circulating: plasma patients with metabolic disease | Human | Perilipin 1 elevated with metab risk factors |
| Amosse et al., 2018 [52] | dUC | Protein arrays/multiplex | Circulating MVs and EXO plasma from metabolic syndrome patients | Human | Adiponectin, adipsin, cathepsin D, CRP, ICAM-1 chemerin, DDP-4 among others. |
| Witczak et al., 2018 [53] | dUC | Immunoassay | Circulating plasma obese before and after bariatric surgery | Human | FABP4 |
| Phoonsawat et al., 2014 [54] | dUC | Immunoblot/ELISA | Circulating: serum obese mice | Mouse | Adiponectin, resistin |