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. 2020 Dec 10;21(24):9417. doi: 10.3390/ijms21249417

Figure 5.

Figure 5

TRPV4 silencing reduces single-cell motility in MDA-MB-468 cells. Immunoblotting studies (A) and densitometry analysis (B) showing TRPV4 siRNA silencing, reducing TRPV4 protein levels by around 90%. **** p < 0.0001 compared with control (unpaired t-test), n = 3, mean ± SD. (C) A collagen-based model and individual cell tracking system were used to assess and quantify the migration of MDA-MB-468 breast cancer cells treated with non-targeting (NT) siRNA (total of 84 cells) and TRPV4 siRNA (total of 72 cells). In this model, the accumulative distance of individual cells migrated over the course of 12 h post-exposure to hypoxia (24 h) is assessed. **** p < 0.0001, (unpaired t-test). Graph represents the mean ± SD for three independent experiments.