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. 2020 Dec 11;10(12):1661. doi: 10.3390/biom10121661

Figure 2.

Figure 2

Overview of the generated constructs. (A) (1) Ty1 monomer, (2) Ty1-dimer generated as described in Figure 1B, (3) Ty1 dimer with PEG linker, generated by performing a click reaction of Ty1-azide and bis-PEG11-DBCO, (4) Ty1-Fc, dimerization through Fc fusion and expression in mammalian 293F cells [27], (5) 4-arm PEG10K Ty1, generated by performing click reaction of Ty1-DBCO to 4-arm PEG10K-azide. (B) Nanobody constructs were loaded on SDS-PAGE and stained using Coomassie G-250. Then 2 ug of each construct was loaded on the gel; molecular weight is indicated on the left (kDa).