Figure 1.
Inhibition of the growth of Glaesserella parasuis by synthetic porcine β-defensin 2 (PBD-2). (a) PBD-2 at various concentrations was incubated with G. parasuis for 1 h before plating and counting the surviving bacteria. Bacterial culture without PBD-2 treatment was used as a negative control. (b) The growth curves of G. parasuis cultured with and without PBD-2 (500 µg/mL) were drawn by recording values of optical density at 600 nm every hour until the stationary phase. The bacterial numbers in mid-log and stationary phases were determined by plate counting. The black-framed white column contains the black column below. Data are presented as mean ± SD and plotted from three independent experiments. *** p < 0.001, **** p < 0.0001, unpaired one-tailed Student′ s t-test.