Figure 5.

Compositional analyses of re-grown multi-species biofilms in the presence (A) and absence (B) of FCS. Biofilms in the “prevention” group as described in Figure 4 were processed for DNA extraction prior to composition analyses via quantitative polymerase chain reaction (qPCR). In brief, biofilms were disrupted, diluted 1:5, then transferred to fresh 6-well culture plates containing chlorhexidine (0.2%), chitosan (0.7 mg/mL), and EDTA (230 mM). Plates were cultured for 24 h to permit biofilm re-growth before DNA was extracted using the DNeasy Extraction Kit, following the manufacturer’s instructions. Average % composition is shown in the bar graphs, data representative of biofilms from three independent repeats (three technical replicates in each experiment).