Figure 6.

Compositional analysis of re-grown multi-species biofilm on bovine dentine discs. Biofilms were grown in RPMI/THB in 24-well plates for 24 h. After incubation, the biofilms were disrupted and diluted to 1:5 and then inoculated on pre-sterilized bovine dentine discs placed in a 24-well plate treated with chitosan at 0.7 mg/mL. Following additional 24 h incubation, the total CFE/mL (A) and composition of the regrown biofilms (B) were assessed using qPCR. Data were analyzed by the Mann–Whitney test. Data representative of n = 3 (**** p < 0.0001).